The six-wheeled vehicle was built to operate for just three months.
But it kept going and going until it was finally doomed by a ferocious dust storm eight months ago.
Flight controllers made numerous attempts to contact it and sent one final series of recovery commands on Tuesday night, accompanied by one last wake-up song, Billie Holiday’s I’ll Be Seeing You. There was no response, only silence.
Remarkably agile until communication ended last June, Opportunity roamed a record 28 miles around Mars.
Opportunity and its long-dead twin rover, Spirit, found evidence that ancient Mars had water flowing on its surface and might have been capable of sustaining microbial life.